James Arnold Taylor
Leonardo (voice)

Mitchell Whitfield
Donatello / Additional Voices (voice)

Nolan North
Raphael / Nightwatcher (voice)

Mikey Kelley
Michelangelo (voice)

Sarah Michelle Gellar
April O'Neil (voice)

Chris Evans
Casey Jones (voice)

Mako
Splinter (voice)

Patrick Stewart
Winters (voice)

Kevin Smith
Diner Cook (voice)

Laurence Fishburne
Narrator (voice)

Zhang Ziyi
Karai (voice)

John DiMaggio
Colonel Santino (voice)

Kevin Michael Richardson
General Aguila / Additional Voices (voice)

Fred Tatasciore
General Gato / Additional Voices (voice)

Dee Bradley Baker
Additional Voices (voice)

Greg Baldwin
Splinter (voice)

Jeff Bennett
Additional Voices (voice)

Jim Cummings
Additional Voices (voice)

Grey DeLisle-Griffin
Additional Voices (voice)

Chris Edgerly
Additional Voices (voice)

Kim Mai Guest
Additional Voices (voice)

Jennifer Hale
Additional Voices (voice)

Jess Harnell
Additional Voices (voice)

Phil LaMarr
Additional Voices (voice)

Paul Michael Robinson
Additional Voices (voice)

Tara Strong
Additional Voices (voice)

Billy West
Additional Voices (voice)

Kevin Munroe
Diner Patron (voice) (uncredited)