Spencer Tracy
Dr. Henry 'Harry' Jekyll / Mr. Hyde

Ingrid Bergman
Ivy Peterson

Lana Turner
Beatrix 'Bea' Emery

Donald Crisp
Sir Charles Emery

Ian Hunter
Dr. John Lanyon

Barton MacLane
Sam Higgins

C. Aubrey Smith
The Bishop

Peter Godfrey
Poole

Sara Allgood
Mrs. Higgins

Frederick Worlock
Dr. Heath

William Tannen
Intern Fenwick

Frances Robinson
Marcia

Denis Greene
Freddie

Billy Bevan
Mr. Weller

Forrester Harvey
Old Prouty

Lumsden Hare
Colonel Weymouth

Lawrence Grant
Dr. Courtland

John Barclay
Constable

Hillary Brooke
Mrs. Arnold (uncredited)

Alec Craig
Tripped Waiter (uncredited)

Brandon Hurst
Dr. Lanyon's Butler Briggs (uncredited)

Olaf Hytten
Hobson (uncredited)

Colin Kenny
Constable (uncredited)

Doris Lloyd
Mrs. Marley (uncredited)

Aubrey Mather
Inspector (uncredited)

Al Ferguson
Constable (uncredited)

Frank Hagney
Drunk (uncredited)

Martha Wentworth
Landlady (uncredited)

Larry Wheat
Church Member (uncredited)

Sailor Vincent
Brawler (uncredited)

Yorke Sherwood
Chairman (uncredited)

Milton Parsons
Choir Master (uncredited)

Claude King
Uncle Geoffrey (uncredited)

Harold Howard
Blind Man (uncredited)

Winifred Harris
Mrs. Weymouth (uncredited)

Douglas Gordon
Cockney (uncredited)

Gwen Gaze
Mrs. French (uncredited)

Bess Flowers
Minor Role (uncredited)

Rita Carlyle
Minor Role (uncredited)

Ted Billings
Bar Patron (uncredited)

Lydia Bilbrook
Lady Copewell (uncredited)

Jimmy Aubrey
Hanger-On (uncredited)