Leonard Nimoy
Self (archive footage)

William Shatner
Self

George Takei
Self

J.J. Abrams
Self

Simon Pegg
Self

Chris Pine
Self

Adam Nimoy
Self

Nichelle Nichols
Self

Zachary Quinto
Self

Zoe Saldaña
Self

Neil deGrasse Tyson
Self

Jim Parsons
Self

Jason Alexander
Self

Mayim Bialik
Self

Walter Koenig
Self

Nicholas Meyer
Self

Bill Prady
Self

Catherine Hicks
Self

Bobak Ferdowsi
Self

Barry Newman
Self

Jeffrey Katzenberg
Self

Karl Urban
Self

James Duff
Self

Brooke Adams
Elizabeth Driscoll (archive footage)

James Arness
Matt Dillon (archive footage)

Jeanne Bal
Nancy Crater (archive footage)

Majel Barrett
Number One (archive footage)

Avery Brooks
Benjamin Sisko (archive footage)

Kaley Cuoco
Penny (archive footage)

Ted Danson
Jack (archive footage)

James Doohan
Scotty (archive footage)

Diana Ewing
Droxine (archive footage)

Terry Farrell
Jadzia Dax (archive footage)

Donald Sutherland
Matthew Bennell (archive footage)

William Windom
Commodore Decker (archive footage)

Tom Selleck
Peter (archive footage)

Liam Neeson
Leo Cutter (archive footage)

Christopher Lloyd
Commander Kruge (archive footage)

Jill Ireland
Leila Kalomi (archive footage)

Jeffrey Hunter
Captain Christopher Pike (archive footage)