Bruce Greenwood
Batman / Bruce Wayne (voice)

Jensen Ackles
Red Hood / Jason Todd (voice)

Neil Patrick Harris
Nightwing (voice)

Jason Isaacs
Ra's al Ghul (voice)

John DiMaggio
Joker (voice)

Wade Williams
Black Mask (voice)

Carlos Alazraqui
Chi Chi / Thug #1 / Baton (voice)

Robert Clotworthy
Leon / Thug (voice)

Gary Cole
Bobo / Commissioner Gordon / Shot / Guard (voice)

Brian George
Ra's Assistant (voice)

Kelly Hu
Ms. Li (voice)

Phil LaMarr
Rick / Bulk (voice)

Vincent Martella
Robin / Jason Todd (voice)

Jim Piddock
Alfred (voice)

Kevin Michael Richardson
Tyler Bramford (voice)

Andrea Romano
Reporter #1 (voice)

Dwight Schultz
Freddie / Drug Dealer (voice)

Fred Tatasciore
Mercenary #1 / Amazo / Guard (voice)

Keri Tombazian
Reporter #2 (voice)

Bruce Timm
The Riddler (voice)

Michael Villani
Reporter #3 (voice)