Milla Jovovich
Alice

Oded Fehr
Carlos Olivera

Ali Larter
Claire Redfield

Iain Glen
Dr. Isaacs

Ashanti
Betty

Christopher Egan
Mikey

Spencer Locke
K-Mart

Matthew Marsden
Slater

Linden Ashby
Chaser

Jason O'Mara
Albert Wesker

Mike Epps
L.J.

Joe Hursley
Otto

James Tumminia
Lab Tech

Madeline Carroll
White Queen

Valorie Hubbard
Ma

Carrie Eklund
Camp Survivor (uncredited)

Brian Steele
Rancid / Tyrant

John Eric Bentley
Umbrella Tech

Kirk B.R. Woller
Scientist

Rick Cramer
Ice Hockey / Corridor Guard

Peter O'Meara
British Envoy

William Abadie
French Envoy

Ramón Franco
Runty

Shane Woodson
Piggy

Geoff Meed
Pock Mark

Rusty Joiner
Eddie

Carolina Jiménez
Mirror Woman

Gary A. Hecker
Tyrant Vocal (voice)

Martin Crewes
Chad Kaplan (uncredited)

Richard Epcar
Additional Voices (voice) (uncredited)

Stefen Hayes
Yuri Loginova (uncredited)

Gary Hudson
Umbrella Captain (uncredited)

Ryan McCluskey
Mr. Grey (uncredited)

Carsten Nørgaard
Zombie Steve (uncredited)

César Ámigo Aguilar
Foreign Envoy (uncredited)