Bob Hoskins
Eddie Valiant

Christopher Lloyd
Judge Doom

Joanna Cassidy
Dolores

Charles Fleischer
Roger Rabbit / Benny The Cab / Greasy / Psycho (voice)

Kathleen Turner
Jessica Rabbit (voice) (uncredited)

Stubby Kaye
Marvin Acme

Alan Tilvern
R.K. Maroon

Richard LeParmentier
Lt. Santino

Lou Hirsch
Baby Herman (voice)

Betsy Brantley
Jessica's Performance Model

Joel Silver
Raoul

Richard Ridings
Angelo

Mike Edmonds
Stretch

Morgan Deare
Editor / Gorilla (voice)

Billy J. Mitchell
Forensic #2

Ed Herlihy
Newscaster

James O'Connell
Conductor

April Winchell
Mrs. Herman (voice)

Mae Questel
Betty Boop (voice)

Mel Blanc
Daffy Duck / Tweety / Bugs Bunny / Sylvester / Porky Pig (voice)

Tony Anselmo
Donald Duck (voice)

Joe Alaskey
Yosemite Sam / Foghorn Leghorn (voice)

David L. Lander
Smart Ass (voice)

Fred Newman
Stupid (voice)

June Foray
Wheezy / Lena Hyena (voice)

Russi Taylor
Minnie Mouse / Birds (voice)

Richard Williams
Droopy (voice)

Pat Buttram
Bullet #1 (voice)

Jim Cummings
Bullet #2 (voice)

Jim Gallant
Bullet #3 (voice)

Frank Sinatra
Singing Sword (voice) (archive sound)

Tony Pope
Goofy / Big Bad Wolf (voice)

Cherry Davis
Woody Woodpecker (voice)

Jack Angel
Additional Voices (voice) (uncredited)

Nancy Cartwright
Dipped Shoe (voice) (uncredited)

Amy Irving
Jessica Rabbit (singing voice) (uncredited)

Mickie McGowan
Mooing Cow / Flying Witch (voice) (uncredited)

Frank Welker
Dumbo (voice) (uncredited)