Barrie Ingham
Basil of Baker Street / Bartholomew (voice)

Vincent Price
Professor Ratigan (voice)

Val Bettin
Dr. David Q. Dawson / Thug Guard (voice)

Candy Candido
Fidget (voice)

Diana Chesney
Mrs. Judson (voice)

Eve Brenner
The Mouse Queen (voice)

Alan Young
Mr. Flaversham (voice)

Basil Rathbone
Sherlock Holmes (voice)

Walker Edmiston
Citizen / Thug Guard (voice)

Wayne Allwine
Thug Guard (voice)

Tony Anselmo
Thug Guard (voice)

Frank Welker
Toby the Dog / Felicia the Cat (voice) (uncredited)

Laurie Main
Dr. Watson (archive sound)

Shani Wallis
Lady Mouse (voice)

Melissa Manchester
Miss Kitty Mouse (voice) (uncredited)