Bud Abbott
Chick Larkin

Lou Costello
Mervin Q. Milgrim

Patric Knowles
Jim Turner

Louise Allbritton
Jane Little

Mary Wickes
Juliet Collins

William Gargan
Detective Inspector Lou

William Bendix
Detective Sgt. Brannigan

Thomas Gomez
Col. J.R. Andrews

Ludwig Stössel
Dr. Anton Marek

Edmund MacDonald
'Murder at Midnight' Announcer

Jerome Cowan
Heller

Walter Tetley
'Shorty', a studio pageboy

Joe Kirk
Harry, 'Murder At Midnight' sound man

Don Porter
'Murder at Midnight' program manager

Norman Abbott
'Murder at Midnight' Organist

Milton Parsons
Coroner (cameo)

Gladys Blake
Telephone Operator

Eddie Bruce
Bill, man telephoning Brazil

Billy Wayne
Jake, man telephoning Alaska

Alice Fleming
Mrs. Laffingwell, dowager telephoning Moscow

Margaret Brayton
Radio Actress (uncredited)

Crane Whitley
Radio Actor (uncredited)

Paul Dubov
Radio Actor (uncredited)

Gene O'Donnell
Radio Actor (uncredited)

Edward Keane
Man in drug store touting Juliet (uncredited)

Duke York
Attendant with the Coroner (uncredited)

Harry Strang
Truck Driver (uncredited)

Bobby Barber
Test Technician in Booth (uncredited)

Frank McLure
Acrobatic Show Spectator (uncredited)