Pete Ploszek
Leonardo

Alan Ritchson
Raphael

Jeremy Howard
Donatello

Noel Fisher
Michelangelo

Megan Fox
April O'Neil

Will Arnett
Vern Fenwick

William Fichtner
Eric Sacks

Danny Woodburn
Splinter

Johnny Knoxville
Leonardo (voice)

Tony Shalhoub
Splinter (voice)

Tohoru Masamune
Shredder

Whoopi Goldberg
Bernadette Thompson

Minae Noji
Karai

Abby Elliott
Taylor

Madison Mason
Councilman

Taran Killam
McNaughton

K. Todd Freeman
Dr. Baxter Stockman

Paul Fitzgerald
Dr. O'Neil

Malina Weissman
Young April O'Neil

Venida Evans
Subway Hostage

Mikal Vega
Sacks Bodyguard

Chris Wylde
Reporter

Chance Kelly
Mr. Rivetti

Rick Chambers
News Anchor

Derek Mears
Dojo Ninja